On this page you will find selection of application reports, scientific research work, tables and helpful tips for the installation and the running of sensors, gas analyzers and analysis systems of BlueSens. This page is still under construction.
Methylobacterium extorquens AM1
Re´mi Peyraud, Patrick Kiefer, Philipp Christen, Jean-Charles Portais, Julia A. Vorholt (2012): ” Co-Consumption of Methanol and Succinate by Methylobacterium extorquens AM1“. Open source article from the US National Library of Medicine National Institutes of Health: http://www.ncbi.nlm.nih.gov/pmc (download the PDF here, 1MB).
Escherichia coli heat shock response
Svenja Lüders, Claas Fallet und Ezequiel Franco-Lara (2009): “Proteome analysis of the Escherichia coli heat shock response under steady-state conditions”. Ein open-source Artikel aus der US National Library of Medicine National Institutes of Health: http://www.ncbi.nlm.nih.gov/pmc/ (Download der PDF-datei hier, Englisch, 670KB)
Pseudomonas putida KT2442
Stéphanie Follonier, Sven Panke and Manfred Zinn (2011): “A reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from gluconate”. Open source article from the US National Library of Medicine National Institutes of Health: http://www.ncbi.nlm.nih.gov/pmc/ (download the PDF here, 1MB).
Pichia pastoris growing at different oxygen levels
Marc Carnicer, Kristin Baumann, Isabelle Töplitz, Francesc Sánchez-Ferrando, Diethard Mattanovich, Pau Ferrer and Joan Albiol (2009):” Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels”. An open source article from the US National Library of Medicine National Institutes of Health: http://www.ncbi.nlm.nih.gov/pmc/ (download here the PDF-file, 1MB).
Pichia pastoris in different oxygen conditions
Baumann, Marc Carnicer, Martin Dragosits, Alexandra B. Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer (2010):” A multi-level study of recombinant Pichia pastoris in different oxygen conditions”. Open source article from the US National Library of Medicine National Institutes of Health: http://www.ncbi.nlm.nih.gov/pmc/ (download the PDF here, 1.3MB)
Continuous H2 production with E. coli
Continuous hydrogen production with E. coli: “Report on the application of BlueSens gas sensor in continuous bioH2 process optimization“. Abstract for the BlueCompetition 2011 by Péter Bakonyi, Nándor Nemestóthy, Katalin Bélafi-Bakó Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Hungary. (Download, PDF-file, 676 KB)
Dr. phys. Janis Kleperis, MSc. Ilze Dimanta, BSc. Arturs Gruduls, MSc. Laimonis Jekabsons, Hydrogen and Gas Sensors Laboratory, Institute of Solid State Physics, University of Latvia: "Biological production of hydrogen by bacterial anaerobic fermentation". (Download, PDF-file, 800KB)
Online biomass estimation with OUR CER calculation
Fed batch cultivation with OUR and CER calculation by Frederik Hoppe and Michael Maurer, University of Applied Sciences FH Campus Vienna and Corinna Rebnegger, University of Natural Resources and Life Sciences, Vienna: "On-line biomass estimation of yeast fed batches using off gas analysis of carbon dioxide and oxygen". (Download, PDF-file, 1.3MB)
Process optimization for the production of antibodies
Florian David, Ezequiel Franco-Lara Technische Universität Braunschweig, Institute of Biochemical Engineering: "Soft-sensor based adapted feed control for rational secretion process optimization". (Download PDF-file, 1.3MB)
Process automation and production optimization Myxobacteria
Abstract of Prof. Dr. Rolf Müller and Dipl.-Ing.(FH) Stephan Hüttel, PhD student at the Department of Pharmaceutical Biotechnology, Saarland University and Helmholtz Institute for Pharmaceutical Research Saarland (HZI): "Application of Gas Analyzers in Process Automation and Production Optimization of Secondary Metabolites from Myxobacteria". (Download PDF-file, 1MB)
Ethanol and CO2/O2 measurements in shake flaks by PD Dr.-Ing. Lars M. Blank, Laboratory of Chemical Biotechnology, Technical University Dortmund: "Monitoring of baker´s yeast fermentations". (Download, PDF-File, 1MB)
Automated DoE with CO2/O2-analysis
Automated DoE with CO2 and O2 analysis by Prof. Dr.-Ing. Reiner Luttmann et al., Research and Transfer Center of Bioprocess Engineering and Analytical Techniques Hamburg University of Applied Sciences "Automated Design of Experiments (DoE) in a multi-bioreactor system BIOSTAT® Qplus 6". (Download, PDF-file, 1MB)
Analysis in the shake flask CO2/O2 sensors
Analysis in the shake flask with CO2 sensor and O2 sensor by Prof. Dr.-Ing. Reiner Luttmann et al., Research and Transfer Center of Bioprocess Engineering and Analytical Techniques Hamburg University of Applied Sciences: "The precultivation in shake flasks for the execution of bioreactor cultivations". (Download PDF-file, 900KB)
OUR/RQ/OTC survey by Prof. Dr.-Ing. Reiner Luttmann et al., Research and Transfer Center of Bioprocess Engineering and Analytical Techniques Hamburg University of Applied Sciences: "Online observation of oxygen uptake and carbon dioxide production and characterisation of oxygen transfer capacity". (Download PDF-file, 1MB)
Analysis of CO2/O2 and ethanol for the production of Biofuel
Analysis of carbon dioxide, ethanol and oxygen for the production of Biofuel. Application report by Dr.-Ing. Eva Maria del Amor Villa, Biochemical Engineering Laboratory, Biochemical and Chemical Department, Technical University Dortmund: "Continuous bio-ethanol production by means of yeast". (Download PDF-File, 740KB)
Application report CO2/O2 analysis
CO2- and O2 analysis in laboratory scale. Application report by DI Dr. Michael Maurer, FH Campus Wien - University of Applied Sciences, Bioengineering degree programme: "Application of a self constructed off gas analyser in the education of bioengineers". (Download PDF-File, 618KB)
CO2 measurement: scientific survey
Application CO2 measurement, scientific survey: "Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440", Andreas Wittgens, Till Tiso, Torsten T Arndt, Pamela Wenk, Johannes Hemmerich, Carsten Müller, Rolf Wichmann, Benjamin Küpper, Michaela Zwick, Susanne Wilhelm, Rudolf Hausmann, Christoph Syldatk, Frank Rosenau and Lars M Blank. (Download, PDF-file, 3MB)
Hydrogen measurement in a photo bioreactor
During the competition "Jugend forscht 2013" Sören Pfitzner und Johannes Wüllenweber under supervision of Dr. Björn Herber produced hydrogen (H2) with Chlamydomonas reinhardtii. (Download PDF-Dokument 1,25MB)
Optimization of Biogas production
Research project for optimization of biogas plants made by Dr. F. Uhlenhut, Prof. Dr. S. Steinigeweg, Prof. Dr. A. Borchert, Prof. Dr. Reiner Lohmüller, University of Applied Sciences Emden/Leer, EUTEC Institute: "Model based optimization of biogas plants". (Download PDF-Datei,Englisch, 840KB)
Comparison BlueSens sensors / Mass specs for OUR and CER calculation
Mathias Aehle, Center for Bioprocess Engineering, Martin-Luther-University Halle-Wittenberg, Halle (Saale) compared BlueSens O2/CO2 analyzers with a mass spec during a cell culture process for the use of OUR and CER calculation "Application of BlueSens® Gas Analyzers in a Cell Culture Process". (Download, Englisch, 1,2 MB)
CO2 measurement in bioreactor off-gas as global pH reference method
Small differences (0.1) in the pH value could have negative impacts on the cells like high lactate production. Therefore a standardized method for pH sensor calibration is extremely important especially for processes that have to be identical all over the world.
In this report Christian Klinger, Roche Diagnostics GmbH, Pharma Biotech Penzberg, presents a calibration method for pH sensors using CO2 measurements of the fermenter off gas to minimize process variabiity.