Analysis system BCpreFerm
For controlled growth in shake flasks and fermenters
BCpreFerm - the whole gas analysis for the scale-up process
Running continuous measurements of the CO2 and O2 contents in shake flasks and fermenters facilitates controlled trial conditions with simultaneous analysis of the metabolic processes. Oxygen or substrate limitations can be detected along with metabolic transpositions. Furthermore, a time specific analysis of the desired product is made possible. For improved comparability, the measurement system BCpreFerm can be used for shake flasks and fermenters.
BCpreFerm consists of:
- up to 6 CO2- sensors
- up to 6 O2- sensors
- Multiplexer BACCom and BACCom EB for connecting 12 sensors through one interface (RS232, USB, Ethernet)
- FermVis data acquisition software, calculation of the OTR, CTR, RQ
Arranging the experiment:
- Fill the culture medium into the shake flask
- Seal the flask with a sterile filter
- Autoclave the filled flask and inoculate it afterwards
- Fix the flask on the shaker
- Place the sensors for CO2 and O2 on the flask
- Start the measurement via the software FermVis
or
- Place the sensors on the flow adapter
- Connect a tube or a pipe
- Start fermentation in the bioreactor
- Start the measurement via the software FermVis
